Journal: Infection and Immunity
Article Title: CpoS-Inc interactions facilitate host cell modulation during Chlamydia trachomatis infection
doi: 10.1128/iai.00548-25
Figure Lengend Snippet: CpoS interacts with Rab GTPases and other C.t . Incs. HeLa cells were transfected with pcDNA 3.1 eGFP-Rab14 or pcMV eGFP Rab 35 and co-infected at an MOI of 5 with C.t. expressing CpoS-HA and either C.t. expressing IPAM-Flag or InaC-Flag. After 24 hpi, CpoS was immunoprecipitated using HA beads, and the elutions were resolved on SDS-PAGE, followed by Western blotting with HA, GFP, and Flag antibodies. Data are representative of three independent experiments.
Article Snippet: HeLa cells, seeded on coverslips, were transfected with GFP-tagged Rab (mentioned above) and Arf GTPases (Addgene #39554, #39556) using Lipofectamine LTX.
Techniques: Transfection, Infection, Expressing, Immunoprecipitation, SDS Page, Western Blot